报告人:   Zhihao Zhuang

Associate Professor

Dept. of Chemistry and Biochemistry

University of Delaware

报告时间：2019年6月14日   上午10:30

报告地点：学院A717

 Interrogation of Protein Ubiquitination in DNA Damage Tolerance

Zhihao Zhuang

Associate Professor

Dept. of Chemistry and Biochemistry

University of Delaware

Posttranslational modification of proteins by ubiquitin (Ub) and ubiquitin-like proteins (Ubls) represents a crucial way of regulating cellular functions. New pathways regulated by ubiquitin and Ubl are being discovered at a fast pace, virtually in every important aspect of biology. Enzymatic ubiquitination usually requires multiple enzymes and faces the problem of poor yield and purity. Chemical ubiquitination circumvents the requirement of the ubiquitin enzyme cascade and can be readily generalized for modifying different target proteins. We developed chemical approaches for efficient mono- and poly-ubiquitination of proteins. Using the chemically ubiquitinated proliferating cell nuclear antigen (PCNA) we uncovered new insights into the regulation of eukaryotic DNA damage tolerance. We have also developed ubiquitin-based probes and inhibitors as well as new assay platforms, allowing us to interrogate the function of deubiquitinases in yeast and human.